IL7R alpha (IL7R) Mouse Monoclonal Antibody [Clone ID: R34-34]

Specifications

Product Data
Clone Name	R34-34
Applications	IF
Recommend Dilution	DDX0700P-50 / DDX0700P-100 Purified: FACS surface, Neutralization (IL7- binding inhibition), Immunoprecipitation. DDX0700A488-50 / DDX0700A488-100 Alexa-fluor®488: Surface Flow cytometry. DDX0700A546-50 / DDX0700A546-100 Alexa-fluor®546: IF. DDX0700A647-50 / DDX0700A647-100 Alexa-fluor®647: Surface Flow cytometry. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human leukemic pre-B cells.
Specificity	Human IL7-Rα.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 546
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 546
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	interleukin 7 receptor
Database Link	Entrez Gene 3575 Human UniProt ID P16871
Background	The IL7-R consists of 2 chains, IL-7Rα known as CD127 and common cytokine receptor γ chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7Rα expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 ; Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54).
Synonyms	Interleukin-7 receptor subunit alpha, IL-7R-alpha, IL7 receptor alpha
Reference Data

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