EGF Mouse Monoclonal Antibody [Clone ID: S-134]
CAT#: DM1012
EGF mouse monoclonal antibody, clone S-134, Purified
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CNY 7,337.00
货期*
4周
规格
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Specifications
Product Data | |
Clone Name | S-134 |
Applications | ELISA |
Recommend Dilution | ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications for Human EGF detection in combination with a monoclonal EGF Tracer (AM09149HR-N / AM09149BT-N, Clone S-146). Suggested Capture Coating Dose: 0.3 µg/well; Substrate: TMB. If the above suggested conditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL of EGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Purified recombinant Human EGF, 6kDa, 53 amino acid residues |
Specificity | This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA. |
Formulation | 0.01M PBS, pH 7.2 without preservatives State: Purified State: Lyophilized purified IgG fraction |
Reconstitution Method | Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/ml |
Concentration | lot specific |
Purification | Affinity Chromatograpghy on Protein G |
Conjugation | Unconjugated |
Storage Condition | Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Gene Name | epidermal growth factor |
Database Link | |
Background | Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development. |
Synonyms | Urogastrone, Epidermal growth factor, URG, HOMG4 |
Reference Data | |
Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane |
Protein Pathways | Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
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