IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: I8-S2]

CAT#: DM1022

IL8 (CXCL8) mouse monoclonal antibody, clone I8-S2, Azide Free



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CNY 7,535.00


货期*
4周

规格
    • 500 ug

Product images

Specifications

Product Data
Clone Name I8-S2
Applications ELISA, FN, WB
Recommend Dilution ELISA: Reacts with human IL-8 (matched pair with clone I8-60, Cat.-No DM1023).
Neutralizing: In chemotaxis assay, the antibody inhibited 97.8% % of the chemotactic activity of IL-8 on RB/293 cell using 5 µg/ml of MAb and 10 ng/ml of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity.
The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS.
Western Blot: Concentration of 0.02-0.1 µg/ml MAb will allow visualization of 100 ng/lane of human IL-8.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Purified recombinant Human IL-8.
Specificity This monoclonal antibody recognizes both recombinant and native Human Interleukin-8.
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Formulation 0.01M PBS, pH 7.0
State: Azide Free
State: Lyophilized purified Ig fraction
Preservative: None
Reconstitution Method Restore with double distillated water to adjust the final concentration to 1.0 mg/ml.
Concentration 1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Condition Upon receipt, store (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name C-X-C motif chemokine ligand 8
Background Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.
Synonyms CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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