S100A4 Mouse Monoclonal Antibody [Clone ID: NJ-4F3]

Specifications

Product Data
Clone Name	NJ-4F3
Applications	ELISA, FC, WB
Recommend Dilution	Flow cytometry: 1.2 µg/106 cells. ELISA: 1/200-1/400. ELISA (capture): Clone NJ-4F3-D1 as detection antibody. ELISA (detection): Clone NJ-5C6-A3 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Genetic immunisation with cDNA encoding S100A4. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
Specificity	Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	S100 calcium binding protein A4
Database Link	Entrez Gene 6275 Human UniProt ID P26447
Background	S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).
Synonyms	S100-A4, CAPL, MTS1, Calvasculin, Metastasin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1
Reference Data

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