CCR4 Mouse Monoclonal Antibody [Clone ID: KH-4F5]

Specifications

Product Data
Clone Name	KH-4F5
Applications	ELISA, FC, IF, WB
Recommend Dilution	Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Flow cytometry: 1.2 µg/106 cells. Immunofluorescence: 1 µg/106 cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunisation with Human CCR4 peptide.
Specificity	Recognizes Human CCR4. Other species not tested.
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine receptor 4
Database Link	Entrez Gene 1233 Human UniProt ID P51679
Background	CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways (2,5).
Synonyms	C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5
Note	SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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