EPCAM Mouse Monoclonal Antibody [Clone ID: MOC-31]

Specifications

Product Data
Clone Name	MOC-31
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 10 µg/ml diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 10 µg/ml. Immunohistochemistry on Paraffin Sections: Antigen retrieval is required using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Lung carcinoma tissue.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the 40kDa cell surface antigen known as CD326.
Formulation	PBS, pH 7.4, Azide Free State: Azide Free State: Liquid purified IgG fraction Stabilizer: None Preservative: None
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Lung cancer is a heterogeneous disease, which can be subdivided into five major tyoes based on histopathological criteria like squamous cell carcinomas, adenocarcinoma, large cell carcinoma, adenosquamous carcinoma and small cell lung cancer (SCLC). A number of important prognostic characteristics have been identified to include the extend of disease and especially for SCLC, tumor sensitivity to chemotherapy. Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Cell Adhesion Molecule (Ep-CAM), Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against CD326, many of which have been described as tumour specific molecules on carcinomas. CD326 is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.