Thrombospondin 1 (THBS1) Mouse Monoclonal Antibody [Clone ID: A6.1]

Specifications

Product Data
Clone Name	A6.1
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil.
Reactivity	Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Specificity	This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum.
Formulation	State: Purified State: Liquid purified Ig fraction Preservative: Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thrombospondin 1
Database Link	UniProt ID P07996
Background	Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Synonyms	TSP, TSP1
Reference Data

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