VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: #EIC]

Specifications

Product Data
Clone Name	#EIC
Applications	ELISA, FC, IF, WB
Recommend Dilution	ELISA: 1-10 µg/ml.  Western Blot: 2-5 µg/ml. FACS Analysis and Cell Sorting: The antibody has been used for the detection of Flt-1 positive cells (e.g. PAE/Flt-1 cells). Immunocytochemistry: 5-30 µg/ml. Immunoprecipitation: 1-5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular Flt-1 D1-D5 (Cat.-No DA3539).
Specificity	The monoclonal antibody will detect Human native and denaturated Human VEGFR-1/ Flt-1 in ELISA experiments, Immunocytochemistry and Cell Sorting. This clone was formerly named FLTEIC.
Formulation	PBS, pH 7.4 containing no preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 1
Database Link	Entrez Gene 2321 Human UniProt ID P17948
Background	Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Synonyms	VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
Reference Data

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