Eng Rat Monoclonal Antibody [Clone ID: MJ7/18]

Specifications

Product Data
Clone Name	MJ7/18
Applications	FC, IHC, IP, WB
Recommend Dilution	ELISA. Western Blot: 2-5 µg/ml. Immuoprecipitation: 1-2 µg. FACS analysis: 3-20 µg/ml. Immunohistochemistry on Frozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine stromal cell line
Specificity	This MJ7/18 Monoclonal antibody reacts with the CD105 molecule, also known as Endoglin.
Formulation	PBS Label: Biotin State: Lyophilized purified IgG fraction of the Culture Supernatant Stabilizer: 50xBSA Preservative: 0.02% Sodium Azide
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein G Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	endoglin
Database Link	Entrez Gene 13805 Mouse UniProt ID Q63961
Background	Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 70-75 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. Endoglin binds TGF-ß1 and TGF-ß3 but not TGF-ß2 efficiently by associating with TGF-ß type II receptor (TßRII). Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.
Synonyms	ENG, END, HHT1, ORW, ORW1
Reference Data

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