LRP2 Mouse Monoclonal Antibody [Clone ID: CD7D5]

Specifications

Product Data
Clone Name	CD7D5
Applications	IF, IHC
Recommend Dilution	Immunofluorescence/Immunohistochemistry: 2-5 µg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Glycoprotein 330 membrane protein fraction of isolated human glomeruli
Specificity	This antibody recognizes Megalin/gp330.
Formulation	PBS without preservatives or stabilizers State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	LDL receptor related protein 2
Database Link	Entrez Gene 4036 Human UniProt ID P98164
Background	Megalin, also called Lipoprotein receptor-related protein-2 (LRP2) or glycoprotein 330, is a 600-kDa transmembrane protein belonging to the LDL-receptor family. Members of this receptor family are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. Megalin was first discovered in 1982 as the pathogenic antigen of Heymann nephritis. The complete cDNA sequences have been characterized for rat and human Megalin. The protein contains a single transmembrane domain, is known to act as an endocytic receptor and is expressed primarily on the apical membrane domain of epithelial cells. Both the protein and its mRNA have been identified in human parathyroid cells, placental cytotrophoblasts and epididymal epithelial cells. The protein is also expressed in mammary epithelia, thyroid follicular cells, yolk sacs, the ciliary body of the eye, the intestinal brush border, the male reproductive tract, uterus and oviduct, and gallbladder epithelium. In addition, immortalised foetal rat alveolar pretype II cells, adult rat type II pneumocytes and human type II cells have all been reported to express megalin. It has been proposed that in adult lung megalin may be important in supplying vitamin E to type II pneumocytes. Specifically, Megalin mediates the uptake of apolipoprotein J, which is a binding protein for the beta-Amyloid peptide, a peptide implicated in Alzheimer's disease. It is also involved in renal uptake of Angiotensin and the reabsorption of various molecules including calcium, vitamin B12 and vitamin D.
Synonyms	Megalin, Glycoprotein 330, LRP-2
Reference Data

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