Pdpn Mouse Monoclonal Antibody [Clone ID: LF3(B7)D5B3]

Specifications

Product Data
Clone Name	LF3(B7)D5B3
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1-5 µg/ml.  Immunofluorescence (Acetone Fixed). Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A membrane protein fraction of isolated Rat glomeruli.
Specificity	This antibody recognizes Rat Podoplanin.
Formulation	PBS, pH 6.0 without preservatives and stabilizers State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for at least 6 month. Avoid repeated freezing and thawing.
Gene Name	podoplanin
Database Link	Entrez Gene 54320 Rat UniProt ID Q64294
Background	Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms	Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025
Note	STAINING PROTOCOL for paraffin-embedded tissue-sections fixed in formalin Anti-rat Podoplanin (#LF3 (B7)D5B3) Ultra Vision LP Large Volume Detection System HRP Polymer (Ready-To-Use) 1. Deparaffinize through xylenes, ethanol, and hydrate to water. 2. Heat-Pre-treatment: in 0,1mol citrate buffer pH 6 in an Autoclave at 1 bar for10 min 3. Cool down at room temperature for 20 min 4. Block in 3% hydrogen Peroxide in PBS 10 min 5. Wash in buffer 6. Apply Ultra V Block and incubate 5 min at room temperature 7. Wash 8. Apply Primary Antibody: LF3 (B7)D5B3 at a dilution 1:1000 in 1%BSA/PBS for 1 hour 9. Wash 4 times 10. Apply Primary Antibody Enhancer and incubate for 10 min at room temperature 11. Wash 4 times 12. Apply HRP Polymer and incubate for 15 min at room temperature in the dark( HRP Polymer is light sensitive) 13. Wash 4 times 14. Incubate with ACE-Chromogen and stain for 5- 10 min 15. Wash 4 times in AD 16. Counter stain with Mayer’s Hämalaun for 1 min 17. Cover slip using an aqueous mounting media
Reference Data

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