MSH6 Mouse Monoclonal Antibody [Clone ID: 44]

Specifications

Product Data
Clone Name	44
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blot: 1/100-1/500. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABC method (30 to 60 minutes at room temperature (requires high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil, colon carcinoma
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic human MSH6 peptide
Specificity	MSH6 is a heterodimer of MSH2 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Liquid purified Ig fraction containing Sodium Azide as preservative.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mutS homolog 6
Database Link	Entrez Gene 2956 Human UniProt ID P52701
Background	An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.
Synonyms	DNA mismatch repair protein Msh6, GTBP
Reference Data

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