Claudin 1 (CLDN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Sections: Dilute to a titer of 1/100-1/200 in an ABC method (incubation period of 30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: breast carcinoma, skin.
Reactivity	Canine, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to C-terminal of Human Claudin 1
Specificity	The antibody recognizes Claudin 1. Cellular localization: Cytoplasmic, cell membrane.
Formulation	State: Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	claudin 1
Database Link	Entrez Gene 9076 Human UniProt ID O95832
Background	Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition.
Synonyms	CLD1, SEMP1, PRO944, UNQ481
Reference Data

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