Leptin (LEP) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA. Immunohistochemistry. Immunoprecipitation. Western Blot.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human Leptin (E. coli).
Specificity	The antibody was raised in rabbits by immunization with the recombinant Human Leptin.
Formulation	0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE State: Aff - Purified State: Lyophilized purified IgG fraction Preservative: None
Reconstitution Method	Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Immunoaffinity chromatography on a column with immobilized Human Leptin
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	leptin
Database Link	Entrez Gene 3952 Human UniProt ID P41159
Background	Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.
Synonyms	LEP, OB, OBS, Obesity factor, Obese protein
Reference Data

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