Hepatitis B X Protein / HBx Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA. (Titer is defined by Indirect ELISA, it is > 1/100,000 for antibody concentration 1 mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve). Western blotting. Immunoprecipitaion.
Reactivity	Hepatitis B Virus
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Hepatitis B Protein X from E. coli.
Specificity	Highly specific for Hepatitis B Protein X.
Formulation	0.05M Phosphate buffer, 0.1M NaCl, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Purification	Immunoaffinity Chromatography on a column with immobilized recombinant Hepatitis B Protein X
Conjugation	Unconjugated
Storage Condition	Store lyophilized antibody at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Background	The recombinant Hepatitis B Protein X is 100% homologous with the natural Hepatitis B Protein X. Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
Synonyms	HBV Protein X
Note	Calculated Molecular Weight: 17 kDa
Reference Data

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