Myostatin Propeptide (MSTN) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	Suitable for ELISA: Titer is defined by indirect ELISA, it is: > 1/100,000 for antibody concentration 1 mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.
Reactivity	Human
Host	Chicken
Clonality	Polyclonal
Immunogen	Recombinant human myostatin propeptide from E. coli.
Specificity	The recombinant human myostatin propeptide is 100% homologous with the human serum myostatin propeptide.
Formulation	0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2 containing no preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 2-8°C for a limited period of time; it does not show decline in activity after two weeks at 2-8°C.
Gene Name	myostatin
Database Link	Entrez Gene 2660 Human UniProt ID O14793
Background	Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.
Synonyms	MSTN, GDF8, GDF-8, Growth differentiation factor 8
Reference Data

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