Lyve1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1-15 µg/ml. Western blot: 2-5 µg/ml. FACS analysis: 3-10 µg/ml.  Immunofluorescence. Immunohistochemistry on Paraffin and Frozen Sections: 0.25-4 µg/ml.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 95%) recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524).
Specificity	Cross reactivity of anti-Mouse Lyve-1 with Rat tissue. The anti-Mouse Lyve-1 polyclonal antibody Cat.-No DP3513P shows a strong cross reaction with Rat Lyve-1 protein.
Formulation	PBS, pH 7.4, without preservatives or stabilizers State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in distilled sterile Water to a concentration of 0.1-1.0 mg/ml.
Purification	Antigen Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 114332 Mouse UniProt ID Q8BHC0
Background	LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms	LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1
Reference Data

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