CD105 (ENG) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA (1-15 µg/ml). Western blot (1-5 µg/ml with the appropriate secondary reagents). FACS analysis and cell sorting (2-5 µg/ml together with the appropriate secondary reagents). Immunofluorescence/Immunohistochemistry (1-5 µg/ml).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human soluble CD105/Endoglin (aa. 22 (Glu) to 586 (Leu)) derived from Insect Cells (Cat.-No DA3523X).
Specificity	This antibody detects CD105.
Formulation	PBS, pH 7.4 without preservatives State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	endoglin
Database Link	Entrez Gene 2022 Human UniProt ID P17813
Background	Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human Endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for Endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.
Synonyms	ENG, END, HHT1, ORW, ORW1
Reference Data

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