Cyclin D1 (CCND1) Rabbit Monoclonal Antibody [Clone ID: SP4]

Specifications

Product Data
Clone Name	SP4
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blot: 1:100 - 1:500. Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:100 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Breast carcinoma
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide from C-terminus of human cyclin D1
Specificity	Reacts to Cyclin D1. Cellular Localization: Nuclear
Formulation	State: Supernatant State: Liquid tissue culture supernatant containing sodium azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin D1
Database Link	Entrez Gene 595 Human UniProt ID P24385
Background	Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Synonyms	Cyclin-D1, PRAD-1 oncogene, BCL-1 oncogene, CCND1, BCL1, PRAD1
Reference Data

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