CD8A Rabbit Monoclonal Antibody [Clone ID: SP16]

Specifications

Product Data
Clone Name	SP16
Applications	IHC
Recommend Dilution	Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:100 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule
Specificity	This antibody recognizes CD8. Cellular Localization: Cell membrane
Formulation	State: Supernatant State: Liquid tissue culture supernatant containing sodium azide as preservative
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8a molecule
Database Link	Entrez Gene 925 Human UniProt ID P01732
Background	CD8 molecule consists of two chains, termed alpha and Beta chain, which are expressed as a disulphide-linked alpha/Beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/Beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not Beta chain).
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway

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