Alkaline Phosphatase (ALPP) Rabbit Monoclonal Antibody [Clone ID: SP15]

Specifications

Product Data
Clone Name	SP15
Applications	IHC
Recommend Dilution	Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:100 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Placenta
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein encoding human Placental Alkaline Phosphatase (PLAP)
Specificity	This antibody reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. This antibody is highly specific to PLAP and shows no cross-reaction with other isoenzymes of alkaline phosphatases. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). Cellular Localization: Cytoplasmic
Formulation	State: Supernatant State: Liquid tissue culture supernatant containing sodium azide as preservative
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alkaline phosphatase, placental
Database Link	Entrez Gene 250 Human UniProt ID P05187
Background	Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
Synonyms	PLAP-1, Alkaline phosphatase Regan isozyme
Reference Data

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