Ifnb1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, Neutralize, WB
Recommend Dilution	Tested Applications: Neutralization Optimal dilutions should be determined by each laboratory for each application. Suggested Applications: ELISA; Western blot; immunoprecipitation; immunohistochemistry Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL. Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-β) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-β provided by the National Institutes of Health [Gb02-902-511]. This material is prepared specifically for effective neutralization of Mu-IFN-β.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant mouse interferon beta (mammalian expressed)
Formulation	0.3 M Sodium Bicarbonate; 0.2 M Sodium Chloride State: Purified
Purification	Protein A purified
Conjugation	Unconjugated
Gene Name	interferon beta 1, fibroblast
Database Link	Entrez Gene 15977 Mouse UniProt ID P01575
Synonyms	IFN-beta, IFNB1, IFB, Fibroblast interferon
Reference Data

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