IL4 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC
Recommend Dilution	Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 0.5-1.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human IL-4 when used with Biotinylated antigen affinity purified anti-Human IL-4 (Cat.-No PP1026B) as the detection antibody at a concentration of approximately 2.0-4.0 µg/ml. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (0.5 ng/ml), a concentration of 3.0-4.5 μg/ml is required. Western blot: Use at a concentration of 0.20-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) E.coli 15.1 KDa derived recombinant Human IL-4 (Cat.-No PA077).
Specificity	Reacts with Human Interleukin-4 / IL4. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 4
Database Link	Entrez Gene 3565 Human UniProt ID P05112
Background	IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Synonyms	IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
Reference Data

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