MCP3 (CCL7) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-3 (100 ng/ml), a concentration of 1-3 µg/ml of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MCP-3 when used with Biotin conjugated anti-Human MCP-3 antibody (cat. PP1046B) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Western Blot: To detect Human MCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Hunan MCP-3.
Specificity	Recognizes Human Macrophage Chemotactic Protein-3 (MCP-3)
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 7
Database Link	Entrez Gene 6354 Human UniProt ID P80098
Background	This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Synonyms	Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28
Reference Data

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