LEC (CCL16) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human LEC (100 ng/ml), a concentration of 1.0-5.0 μg/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 µl/well), a concentration of 1-2 µg/ml of this antibody will detect at least 0.03 ng/well of recombinant human LEC when used with biotinylated antigen affinity purified anti-human LEC as the detection antibody at a concentration of at least 1 µg/ml. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) recombinant Human LEC
Specificity	This antibody recognises Human LEC.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 16
Database Link	Entrez Gene 6360 Human UniProt ID O15467
Background	LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.
Synonyms	C-C motif chemokine 16, Chemokine LEC, Liver-expressed chemokine, Monotactin-1, Chemokine CC-4, LCC1, LCC-1, NCC-4, ILINCK, SCYA16
Reference Data

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