MCP4 (CCL13) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-4 (100 ng/ml), a concentration of 0.8-1.0 µg/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-4 when used with Biotin anti-human MCP-4 antibody (cat. AP01117BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Western Blot: To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) recombinant Human MCP-4
Specificity	Recognises Human MCP-4.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 13
Database Link	Entrez Gene 6357 Human UniProt ID Q99616
Background	Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.
Synonyms	C-C motif chemokine 13, CCL13, MCP4, NCC1, SCYA13, CK-beta-10, NCC-1, Monocyte chemotactic protein 4
Reference Data

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