Csf2 Rabbit Polyclonal Antibody
CAT#: PP003P2
Csf2 rabbit polyclonal antibody, Aff - Purified
Conjugation: Biotin
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CNY 4,790.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommend Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a oncentration of 0.012-0.02 µg/ml of this antibody is required. Indirect ELISA: To detect Mouse GM-CSF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse GM-CSF. Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine GM-CSF (PP003B1 or PP003B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. Western Blot: To detect Mouse GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | E.coli derived Highly pure (>98%) recombinant Murine GM-CSF |
Specificity | This antibody reacts with Mouse GM-CSF. |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography employing immobilized mGM-CSF matrix. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | colony stimulating factor 2 (granulocyte-macrophage) |
Database Link | |
Background | The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia. |
Synonyms | CSF2, GMCSF, Sargramostim, Molgramostin |
Note | Centrifuge vial prior to opening! |
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