Il7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Murine IL-7 by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Murine IL-7. Sandwich ELISA: To detect Murine IL-7 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine IL-7 (Cat.-No PP013P1 or PP013P2) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Murine IL-7. Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.ocli derived recombinant Mouse IL-7 (Cat.-No PA014).
Specificity	This antibody reacts with Mouse Interleukin-7 (IL-7). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified Ig fraction Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 7
Database Link	Entrez Gene 16196 Mouse UniProt ID P10168
Background	Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
Synonyms	IL-7
Note	Centrifuge vial prior to opening!
Reference Data

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