Cntf Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA Direct: To detect Rat CNTF by indirect ELISA (using 100 ml/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 ml/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. The Biotinylated antibody in conjunction with Anti-Rat CNTF (PP030P1 or PP030P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified recombinant CNTF.
Specificity	This antibody recognises Rat CNTF.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ciliary neurotrophic factor
Database Link	Entrez Gene 25707 Rat UniProt ID P20294
Background	CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.
Synonyms	Ciliary Neurotrophic Factor
Reference Data

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