MCP1 (CCL2) Rabbit Polyclonal Antibody
CAT#: PP037P2
MCP1 (CCL2) rabbit polyclonal antibody, Aff - Purified
Conjugation: Biotin
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CNY 4,790.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommend Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rMCP-1 (50 ng/ml), a concentration of 1.75-3.0 µg/ml of this antibody is required. ELISA: To detect rMCP-1 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant rMCP-1. Western Blot: To detect rMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMCP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF). |
Specificity | This antibody recognises Rat MCP-1(MCAF). |
Formulation | PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity chromatography. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | C-C motif chemokine ligand 2 |
Database Link | |
Background | This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
Synonyms | C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11 |
Note | Centrifuge vial prior to opening! |
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