G CSF (CSF3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human G-CSF (0.5 ng/ml), a concentration of 0.04-0.06 µg/ml of this antibody is required. Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (Cat.-No PP1011B1 or PP1011B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human G-CSF. Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.25 µg/ml with an Overnight Incubation at 4°C. This antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human angiosarcoma. An HRP-labeled polymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060).
Specificity	Recognizes Human Granulocyte Colony Stimulating Factor (G-CSF). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized hG-CSF matrix.
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 3
Database Link	Entrez Gene 1440 Human UniProt ID P09919
Background	Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
Synonyms	CSF3, GCSF, Pluripoietin, C17orf33
Note	Centrifuge vial prior to opening.
Reference Data

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