IL9 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-9 (5.0 ng/ml), a concentration of 1.0-2.0 µg/ml of this antibody is required. Sandwich ELISA: To detect Human IL-9 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti-Human IL9 antibody (PP1031B1 or PP1031B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%), E.coli derived 14.0 kDa recombinant Human IL-9
Specificity	This antibody detects Interleukin-9.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 9
Database Link	Entrez Gene 3578 Human UniProt ID P15248
Background	Interleukin 9 (IL9) is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL9 stimulates cell proliferation and prevents apoptosis. The IL9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma.
Synonyms	IL-9, T-cell growth factor P40, P40 cytokine
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Asthma, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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