M-CSF (CSF1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02-0.03 µg/ml of this antibody is required. Indirect ELISA: To detect hM-CSF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Sandwich ELISA: To detect hM-CSF by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (Cat.-No PP1048B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.5 µg/ml with an overnight incubation at 4°C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human cervical squamous cell carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommneded.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human M-CSF (hM-CSF) (Cat.-No PA102)
Specificity	Recognizes Human Macrophage Colony Stimulating Factor (M-CSF). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 1
Database Link	Entrez Gene 1435 Human UniProt ID P09603
Synonyms	CSF1, MCSF, Macrophage colony-stimulating factor 1, Macrophage Colony Stimulating Factor
Note	Centrifuge vial prior to opening!
Reference Data

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