PDGF AA (PDGFA) (PDGF-AA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/ml), a concentration of 0.05 - 0.07 µg/ml of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at 1.0 µg/ml overnight at 4°C. (A HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μl/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required; allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μl/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required; in conjunction with a biotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hPDGF-AA
Specificity	The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.
Formulation	PBS, pH 7.2, without preservatives State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Immunoaffinity chromatography using immobilized human PDGF matrix
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at -20°C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8°C. Frozen aliquots are stable for six months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	platelet derived growth factor subunit A
Database Link	Entrez Gene 5154 Human UniProt ID P04085
Synonyms	Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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