LTA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: (Direct): To detect hTNF-β by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hTNF-beta.
Specificity	This antibody reacts with Tumor Necrosis Factor beta.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lymphotoxin alpha
Database Link	Entrez Gene 4049 Human UniProt ID P01374
Synonyms	TNFB, TNFSF1, LTA, LT-alpha
Note	Centrifuge vial prior to opening!
Reference Data

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