VEGFA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human VEGF by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at ~1.0 µg/ml. This Biotin conjugated antiboy allows the detection of 0.2-0.4 ng/well of recombinant Human VEGF. Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antiboy in conjunction with purified Cat.-No PP1073P as Capture antibody allows the detection of 2000-4000 pg/ml of recombinant Human VEGF. Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human VEGF (Cat.-No PA136).
Specificity	This antibody reacts with Human Vascular Endothelial Growth Factor. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor A
Database Link	Entrez Gene 7422 Human UniProt ID P15692
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Note	Centrifuge vial prior to opening!
Reference Data

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