GRO alpha (CXCL1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of Human GRO/MGSA (100 ng/ml), a concentration of 1.5-3.0 µg/ml of this antibody is required. Sandwich ELISA: To detect Human GRO/MGSA by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti Human GRO/MGSA (Cat.-No PP1080B1 or PP1080B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO/MGSA. Western blot: To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Frozen and Paraffin Embedded Sections: 0.125-0.150 μg/ml with an overnight incubation at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of Human colon/rectum adenocarcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human GRO/MGSA (Cat.-No PA062).
Specificity	This antibody is specific for Human Growth Related Oncoprotein alpha. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography immobilized Human GRO/MGSA matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 1
Database Link	Entrez Gene 2919 Human UniProt ID P09341
Background	Three Human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.
Synonyms	GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, MGSA, NAP-3, GRO-alpha(1-73)
Note	Centrifuge vial prior to opening!
Reference Data

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