Osteoprotegerin (TNFRSF11B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human OPG by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5–2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti-Human OPG antibody (Cat.-No PP1107B1 or PP1107B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human OPG. Western Blot To detect Human OPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained Formalin-Fixed Paraffin-Embedded Sections of Human breast invasive ductal carcinoma. Use at 0.5 µg/ml with overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified (> 98%) E. Coli derived recombinant Human OPG (Cat.-No PA159)
Specificity	Recognizes Human Osteoprotegerin (hOPG). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized hOPG matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor receptor superfamily member 11b
Database Link	Entrez Gene 4982 Human UniProt ID O00300
Background	Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
Synonyms	OPG, OCIF
Note	Centrifuge vial prior to opening!
Reference Data

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