Igf1 Goat Polyclonal Antibody
CAT#: PP1120P1
Igf1 goat polyclonal antibody, Aff - Purified
Conjugation: Biotin
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CNY 3,910.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommend Dilution | Neutralization: To yield one half-maximal inhibition [ND50] of the biological activity of mIGF-I (200 ng/ml), a concentration of 12-14 μg/ml of this antibody is required. Indirect ELISA: To detect Murine IGF-I by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I. Sandwich ELISA: To detect Murine IGF-I by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IGF-I (PP1120B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IGF-I. Western Blot: To detect Murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Mouse |
Host | Goat |
Clonality | Polyclonal |
Immunogen | E.coli derived highly pure (> 98%) recombinant Murine IGF-I. |
Specificity | Reacts with Insulin-like Growth Factor I (IGF-I). |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody prior to reconstitution at -20°C for one year. Following reconstitution the antibody can be stored at 2-8°C for 2 weeks. Avoid repeated freezing and thawing. |
Gene Name | insulin-like growth factor 1 |
Database Link | |
Background | Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth. |
Synonyms | IGF-I, IBP1, Somatomedin |
Note | Centrifuge vial prior to opening! |
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