Igf1 Goat Polyclonal Antibody

CAT#: PP1120P1

Igf1 goat polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 3,910.00


货期*
5周

规格
    • 50 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommend Dilution Neutralization: To yield one half-maximal inhibition [ND50] of the biological activity of mIGF-I (200 ng/ml), a concentration of 12-14 μg/ml of this antibody is required.
Indirect ELISA: To detect Murine IGF-I by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I.
Sandwich ELISA: To detect Murine IGF-I by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IGF-I (PP1120B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IGF-I.
Western Blot: To detect Murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Goat
Clonality Polyclonal
Immunogen E.coli derived highly pure (> 98%) recombinant Murine IGF-I.
Specificity Reacts with Insulin-like Growth Factor I (IGF-I).
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody prior to reconstitution at -20°C for one year.
Following reconstitution the antibody can be stored at 2-8°C for 2 weeks.
Avoid repeated freezing and thawing.
Gene Name insulin-like growth factor 1
Background Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
Synonyms IGF-I, IBP1, Somatomedin
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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