DEFB4B Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, Neutralize, WB
Recommend Dilution	Sandwich ELISA: To detect Human BD-2 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated Anti-Human BD-2 (Cat.-No PP1125B1 or PP1125B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-2. Western Blot: To detect Human BD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BD-2 (1.0 μg/ml), a concentration of 5.0-8.0 mg/ml of this antibody is required.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human BD-2 (Cat.-No PA139)
Specificity	This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Immunoaffinity Chromatography employing immobilized hBD-2 matrix.
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	defensin beta 4B
Database Link	Entrez Gene 100289462 Human UniProt ID O15263
Background	This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
Synonyms	Beta-defensin 4A, Beta-defensin 2, BD-2, hBD-2, DEFB102, DEFB2, Skin-antimicrobial peptide 1, DEFB4B, DEFB4A
Note	Molecular Weight: ~150 kDa
Reference Data

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