Il1a Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Immunohistochemistry: we recommend use of 1 µg per ml as a starting dilution. Paraffin sections may require pretreatment like Proteinase K or Trypsin predigestion to improve staining. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1 alpha (50 pg/ml), a concentration of 0.011-0.017 µg/ml of this antibody is required. ELISA: To detect rIL-1 alpha by direct ELISA (using 100 ml/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant rIL-1 alpha. Western blot: To detect rIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-1 alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Recombinant rat IL-1 alpha
Specificity	This antibody reacts with Rat Interleukin 1 alpha
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 1 alpha
Database Link	Entrez Gene 24493 Rat UniProt ID P16598
Background	Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Synonyms	IL-1 alpha, IL1F1, IL1A, Hematopoietin-1
Note	Centrifuge vial prior to opening!
Reference Data

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