BAFF (TNFSF13B) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Neutralization. ELISA: To detect hBAFF by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hBAFF. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hBAFF.
Formulation	0.5 X PBS, pH 7.4 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of > 0.2 mg/ml.
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor superfamily member 13b
Database Link	Entrez Gene 10673 Human UniProt ID Q9Y275
Background	Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.
Synonyms	TNFSF13B, BLYS, TALL1, TNFSF20, ZTNF4
Reference Data

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