Galectin 1 (LGALS1) Rabbit Polyclonal Antibody

CAT#: PP1223B1

Galectin 1 (LGALS1) rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 3,910.00


货期*
5周

规格
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Direct ELISA: To detect Human Galectin-1 by direct ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-1.
Sandwich ELISA: To detect Human Galectin-1 by Sandwich ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody, in conjunction with Purified anti-Human Galectin-1 antibody (PP1223P) as Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-1.
Western blot: To detect Human Galectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen E.coli derived recombinant Human Galectin-1.
Specificity Reacts with Galectin-1.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS solution containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography.
Conjugation Biotin
Storage Condition Store the antibody prior to reconstitution at -20°C.
Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name galectin 1
Background The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.
Synonyms LGALS1, Galectin1, Lectin galactoside-binding soluble 1, Beta-galactoside-binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, MAPK-activating protein PM12
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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