CDK1 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1:500 to 1:2000. Immunoprecipitation. Immunohistochemistry on paraffin sections: 1:200 to 1:1000. Western Blot: 1:500 to 1:1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	cdc2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 34 kDa cdc2 protein. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdc2 from other species may also occur.
Formulation	State: Serum State: Liquid purified Ig containing 0.01% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase 1
Database Link	Entrez Gene 983 Human UniProt ID P06493
Background	The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Synonyms	CDK1, CDC2, CDC28A, CDKN1, P34CDC2, p34 protein kinase
Reference Data

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