Collagen IV (COL4A1) Rabbit Polyclonal Antibody
CAT#: R1041
Collagen IV (COL4A1) rabbit polyclonal antibody, Aff - Purified
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CNY 8,194.00
货期*
5周
规格
Cited in 6 publications. |
Specifications
Product Data | |
Applications | ELISA, IHC, IP, WB |
Recommend Dilution | Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/50,000. Western blot: 1/1,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200. QC: This product was assayed by Immunoblot and found to be reactive against Collagen IV at a dilution of 1/5,000-1/10,000. This product was also assayed against 1.0 µg of Collagen IV in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat.-No R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock concentration showed best results. |
Reactivity | Bovine, Human, Mammalian |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Collagen type IV purified from Human and Bovine placenta. |
Specificity | This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
Formulation | 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
Concentration | lot specific |
Purification | Immunoaffinity Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. For extended storage, mix with an equal volume of glycerol. Avoid repeated freezing and thawing. |
Gene Name | collagen type IV alpha 1 chain |
Database Link | |
Background | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens were extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. |
Synonyms | COL4A1 |
Reference Data |
Citations (6)
The use of this Antibodies has been cited in the following citations: |
---|
The newly established bovine endometrial gland cell line (BEGC) forms gland acini in vitro and is only IFNτ-responsive (MAPK42/44 activation) after E 2 and P 4 -pre-incubation.
,null,
Placenta
,PubMed ID 29941175
[COL4A1]
|
IFNτ mediates chemotaxis, motility, metabolism and CK18 downregulation in bovine trophoblast cells in vitro via STAT1 and MAPK42/44 signaling.
,null,
Placenta
,PubMed ID 29626977
[COL4A1]
|
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
,Rodriguez-Teja M, Breit C, Clarke M, Talar K, Wang K, Mohammad MA, Pickwell S, Etchandy G, Stasiuk GJ, Sturge J,
J Vis Exp
,PubMed ID 27684203
[COL4A1]
|
AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival
,Rodriguez-Teja M, Gronau JH, Breit C, Zhang YZ, Minamidate A, Caley MP, McCarthy A, Cox TR, Erler JT, Gaughan L, Darby S, Robson C, Mauri F, Waxman J, Sturge J,
J. Pathol.
,PubMed ID 25408555
[COL4A1]
|
The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens
,Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, Bruckner-Tuderman L, Bruckner P, Hansen U,
J. Biol. Chem.
,PubMed ID 22493504
[COL4A1]
|
Perlecan is critical for heart stability
,Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, Fan Y, Kolbe T, Breitbach M, Addicks K, Welz A, Brem G, Hescheler J, Aszodi A, Costell M, Bloch W, Fleischmann BK,
Cardiovasc. Res.
,PubMed ID 18694874
[COL4A1]
|
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