Collagen VI (COL6A1) Rabbit Polyclonal Antibody
CAT#: R1043B
Collagen VI (COL6A1) rabbit polyclonal antibody, Biotin
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CNY 8,363.00
货期*
4周
规格
Cited in 1 publication. |
Specifications
Product Data | |
Applications | ELISA, IHC, IP, WB |
Recommend Dilution | ELISA: 1/10,000-1/50,000. Immunohistochemistry: 1/200-1/1,000. Immunoprecipitation: 1/100. Western Blot: 1/5,000-1/10,000. |
Reactivity | Bovine, Human, Mammalian |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Collagen type VI purified from Human and Bovine placenta. |
Specificity | Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
Formulation | 0.125M Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 Label: Biotin State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG molecule |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent) |
Concentration | lot specific |
Purification | Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | collagen type VI alpha 1 |
Database Link | |
Background | The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. |
Synonyms | COL6A1 |
Reference Data | |
Protein Pathways | ECM-receptor interaction, Focal adhesion |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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A microRNA network regulates proliferative timing and extracellular matrix synthesis during cellular quiescence in fibroblasts
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Genome Biology
,PubMed ID 23259597
[COL6A1]
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