Collagen VI (COL6A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/10,000-1/50,000. Immunohistochemistry: 1/200-1/1,000. Immunoprecipitation: 1/100. Western Blot: 1/5,000-1/10,000.
Reactivity	Bovine, Human, Mammalian
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen type VI purified from Human and Bovine placenta.
Specificity	Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation	0.125M Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 Label: Biotin State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent)
Concentration	lot specific
Purification	Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen type VI alpha 1
Database Link	Entrez Gene 1291 Human UniProt ID P12109
Background	The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
Synonyms	COL6A1
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion

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