Alkaline phosphatase / ALPI / IAP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alkaline phosphatase from calf intestine
Specificity	This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P19111
Synonyms	Intestinal alkaline phosphatase
Reference Data

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