ALDOA Goat Polyclonal Antibody
CNY 20,681.00
货期*
4周
规格
Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most Immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of Aldolase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate. |
Reactivity | Human, Rabbit |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Aldolase [Rabbit Muscle]. |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. State: Serum State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Prepared from monospecific antiserum by a delipidation and defibrination. |
Conjugation | Unconjugated |
Storage Condition | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
Database Link | |
Synonyms | ALDA, NY-LU-1, Muscle-type aldolase |
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