ALDOA Goat Polyclonal Antibody
CAT#: R1054BS
ALDOA goat polyclonal antibody, Biotin, Purified
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | Western blot:1/500-1/5,000. Immunoprecipitation: 1/100. Use approximately 5 µl of antibody to immunoprecipitate 50 µl of protein lysate. ELISA: 1/5,000-1/20,000. This antibody has been assayed against 1.0 µg of aldolase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product. |
Reactivity | Human, Rabbit |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Aldolase from rabbit muscle |
Specificity | This antibody detects rabbit aldolase. Cross reactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction. Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Database Link | |
Synonyms | ALDA, NY-LU-1, Muscle-type aldolase |
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