ALDOA Goat Polyclonal Antibody

CAT#: R1054BS

ALDOA goat polyclonal antibody, Biotin, Purified



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CNY 7,688.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution Western blot:1/500-1/5,000.
Immunoprecipitation: 1/100.
Use approximately 5 µl of antibody to immunoprecipitate 50 µl of protein lysate.
ELISA: 1/5,000-1/20,000.
This antibody has been assayed against 1.0 µg of aldolase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
Reactivity Human, Rabbit
Host Goat
Clonality Polyclonal
Immunogen Aldolase from rabbit muscle
Specificity This antibody detects rabbit aldolase. Cross reactivity against aldolase from other tissues and species may also occur.
Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [rabbit muscle].
It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized purified Ig fraction.
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Synonyms ALDA, NY-LU-1, Muscle-type aldolase
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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