Bilirubin oxidase Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Bilirubin oxidase [Myrothecium verrucaria] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.
Reactivity	Myrothecium
Host	Goat
Clonality	Polyclonal
Immunogen	Bilirubin oxidase from Myrothecium verrucaria
Specificity	This antibody detects Bilirubin oxidase [Myrothecium verrucaria]. Cross reactivity against Bilirubin oxidase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Bilirubin oxidase [Myrothecium verrucaria].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q12737
Background	Bilirubin Oxidase is an enzyme that catalyses the oxidation of bilirubin to biliverdin and other substrates. This enzyme has been expected to be used in the field of clinical pathology for the enzymatic determination of total bilirubin in serum and the treatment of jaundice and hyperbilirubinemia.
Reference Data

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